John Wirthlin Hickey

Assistant Professor of Biomedical Engineering

The Hickey Lab sits at the interface of engineering and immunology, using and developing systems immunology tools to investigate tissue structure in situ. We also use multiplexed imaging and computational techniques to characterize spatial cellular responses related to the effectiveness of anti-cancer cell or biomaterial therapies.  John has received a number of awards for his work, including the NSF Graduate Research Fellowship, ARCS Scholar, Siebel Scholar, NCI Postdoctoral Fellowship, and American Cancer Society Postdoctoral Fellowship.

Appointments and Affiliations

  • Assistant Professor of Biomedical Engineering
  • Assistant Professor in Biostatistics & Bioinformatics
  • Member of the Duke Cancer Institute

Contact Information

Education

  • Ph.D. Johns Hopkins University, School of Medicine, 2019

Research Interests

Using and developing systems biology tools and technologies to describe and control spatial relationships between cells in tissues, particularly in cell therapies.

Awards, Honors, and Distinctions

  • American Cancer Society Postdoctoral Fellowship. American Cancer Society. 2020

Courses Taught

  • EGR 393: Research Projects in Engineering
  • BME 792: Continuation of Graduate Independent Study
  • BME 791: Graduate Independent Study
  • BME 590: Special Topics in Biomedical Engineering
  • BME 493: Projects in Biomedical Engineering (GE)
  • BME 394: Projects in Biomedical Engineering (GE)
  • BME 260L: Modeling Cellular and Molecular Systems

In the News

Representative Publications

  • Strasser, Michael K., David L. Gibbs, Philippe Gascard, Joanna Bons, John W. Hickey, Deng Pan, Joseph A. Caruso, et al. “Concerted changes in Epithelium and Stroma: a multi-scale, multi-omics analysis of progression from Barrett's Esophagus to adenocarcinoma.” Developmental Cell 60, no. 20 (October 2025): 2807-2824.e7. https://doi.org/10.1016/j.devcel.2025.06.034.
  • Border, Samuel P., Ricardo Melo Ferreira, Nicholas Lucarelli, Suhas Katari Chaluva Kumar, Anindya S. Paul, David Manthey, Laura Barisoni, et al. “FUSION: a web-based application for in-depth exploration of multi-omics data with brightfield histology.” Nat Commun 16, no. 1 (September 25, 2025): 8388. https://doi.org/10.1038/s41467-025-63050-9.
  • Jain, Yashvardhan, Jodie Jepson, Roy Chen, Elizabeth Maier, Bruce W. Herr, Aleix Puig-Barbe, Ellen M. Quardokus, et al. “Exploring endothelial cell environments across organs in spatially resolved omics data.” BioRxiv, September 25, 2025. https://doi.org/10.1101/2025.09.23.678129.
  • Bialy, Nikki, Frank Alber, Brenda Andrews, Michael Angelo, Brian Beliveau, Lacramioara Bintu, Alistair Boettiger, et al. “Harmonizing the Generation and Pre-publication Stewardship of FAIR bioimage data.” ArXiv, September 4, 2025.
  • Nejatfard, Anahita, John H. Klich, Noah Eckman, Sophia J. Bailey, Kyra Gillard, Daniel Ramos Mejia, Ben S. Ou, Jerry Yan, John W. Hickey, and Eric A. Appel. “A Transient Immunostimulatory Niche Synergizes Adoptive and Endogenous Immunity for Enhanced Tumor Control.” BioRxiv, August 25, 2025. https://doi.org/10.1101/2025.08.22.671875.